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Fastest route: the Duskwire rod procs Darkened at 68% - get it from Hollow's quest in the Underground Music Venue (catch a Catfish, hand it in, buy the rod for ~1.75M C$; level 275 required). No Duskwire? The Noir enchantment rolls 50%.

Every method, ranked

MethodChanceHow
Mastery Enhancement (underground)100%With Mastery Enhancement active while fishing underground
Duskwire rod68%Hollow quest reward - Underground Music Venue
Noir enchantment50%Random roll from a regular Enchant Stone - works on any rod
Blackout admin event25%60-second admin-triggered event - fish fast while it's live
Depthseeker rod25%Rod passive
Classic Commotion event~9%Admin event
Natural catch1.09%Any fish, anywhere, no setup
Appraisal~0.67%Appraising a caught fish at the appraiser

For the Oppressor quest

The Oppressor rod questline asks you to submit a Darkened Enchant Relic - an Enchant Relic carrying the Darkened mutation, not just any dark fish. Target-farm Enchant Relics with the Duskwire equipped (68% mutation rate beats every other method by a mile), or roll Noir onto a rod and fish relic spots until one lands black.

FAQ

Does Darkened stack with other boosts?

It's a mutation multiplier (1.5x sell value) and pairs with weight/quality - the all-black visual is just the tell.

Best free method?

Blackout events when admins run them (25% with zero gear) - otherwise grinding natural catches at 1.09% is painfully slow.